Optimization of mixing parameters in lab automation for high & low sample volumes

Feb 202012
Optimization of mixing parameters in lab automation for high & low sample volumes

In the past the significance of microplate mixing has mainly been ignored as a serious problem, although potentially it could undermine a generation of meaningful data.
We analyzed orbital microplate mixing technologies to get a better understanding of the current requirements for mixing technologies that can be applied to compounds or bioassays in microplates.
This article reviews some results of studies with BioShake orbital plate shaker, such as where the greatest need for improved mixing of high sample volume in standard tubes against low sample volume in HTS microplates.
It also discusses the influences of construction material, shape and well volume of the sample carrier to the mixing results depending on the physical properties of the liquids.

Optimization of Mixing Processes in Microplates

Jan 272011
Optimization of Mixing Processes in Microplates

A Methodology and Study of Microplate Mixing Techniques Including BioShake 5000

Despite the great interrelation of a fast and complete mixing of assay components to the results, these procedures received only a very limited interest in laboratory routine. Due to the trend of ever increasing miniaturization in biotechnology the usual sample volumes decreases rapidly.

As a consequence, a insufficient mixing has an unfavourable effect to the quality of the results in a manner never before seen.

For simultaneous processing of samples microplates are used as a laboratory standard tool. To achieve a highly effective mixing it is essential to supply enough energy for generating a macroscopic flow in the fluid. Several established methods for generating mixing effects in microplates are shown in Figure 1. A major disadvantage of all methods with contact to the sample is the serious risk of contamination and falsification of results.

BioShake 3000 – Redesigned Version 2011

Dec 182010
BioShake 3000 – Redesigned Version 2011

The universal orbital shaker for robots.

Q.Instruments BioShake 3000 offers a high-speed shaking system with powerful shaking movements from 200 rpm up to 3,000 rpm, with an fixed amplitude of 2.0 mm.
German designed and manufactured, BioShake 3000 includes an, ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.

An automatic control of acceleration enables a slow or quick start without spilling of samples. It is ideally suited for 96, 384 and 1536 microtiter plates, tubes and glass vials. It is completely equipped for hands-free operation with an extensive remote command set for easy software integration.

BioShake iQ - New Version 2010 - New Adapters

Jul 292010
BioShake iQ - New Version 2010 - New Adapters

SMALL . LIGHT . EFFICIENT . QUIET

The ultra-flat micro thermo shaker with patented 3D-Shake-Control and Anti-Vibration technology supports accurate and efficient mixing on the microliter scale of a wide range of SBS-sized 96-, 384-, and 1536-well microplates, tubes, and glass vials
The vibration-free shaking movements are remote controlled from 200 to 3000 rpm.

Compact and stylishly designed, the BioShake orbital shaker lets you perform all your standard runs with a minimum of adjustments, and offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.

By combining the mixing operation with the incubation phase, reaction process times and operator workload are reduced and efficiency of many procedures is increased, resulting in a higher throughput.

Product Focus: Biological Shakers - Published in Lab Manager Magazine

Jun 302010
Product Focus: Biological Shakers - Published in Lab Manager Magazine

Published in Lab Manager Magazine, by Angelo DePalma
www.labmanager.com/articles.asp?ID=443
From Lab Product News, Published: 1/6/2010

Biological Shakers: Are you using a biological shaker in your lab? Are you considering purchasing a biological shaker soon? Lab Manager Magazine’s online surveys help improve the purchasing process and provide you with greater confidence in your final purchasing decision.

Biological shakers are instruments used to agitate a collection of biological samples simultaneously. Shakers consist of a motor attached to a flat surface, with fasteners for securing labware whose contents require mixing. All points on the surface move in the same fashion within the x-y plane, either back and forth (reciprocal shakers) or in a circular motion (orbital shakers)….

MICROPLATE MIXING, bioassay panacea or of unproven distraction?

Jun 092010
MICROPLATE MIXING, bioassay panacea or of unproven distraction?

By Dr John Comley .  Winter 2007/2008 DDW – Drug Discover World … Despite general awareness of the significance of microplate mixing, it is still a relatively low priority in most laboratories. Microplate mixing has largely been ignored as a serious problem, although potentially it could undermine the generation of meaningful data. In September 2007, [...]

BioShake iQ - Optimized temperature homogeneity while heating processes

Apr 212010
BioShake iQ - Optimized temperature homogeneity while heating processes

The BioShake thermomixers are perfectly suitable for applications in calibration laboratories and conform to the requirements specified by DIN ISO 9001:2000. The instruments provide highest temperature accuracy to ±0.1 °C and temperature uniformity better than ± 1.0 °C across the heating surface. Reference temperature measurements can be carried out with integrated additional precision reference sensor. [...]

BioShake - A guide for the changing of adapter plates

Mar 182010
BioShake - A guide for the changing of adapter plates

To change the adapter plate please follow the subsequent steps: NOTE: If the security cover plate (thickness 1 mm) or an adapter plate already be mounted, please remove it first! Turn Off the instrument! Remove all sample carriers B-3 (tubes, vials, microplates, etc.). Loosen the 3 torx screws A-1, A-2 and A-3 by using the [...]

BioShake 3000-T elm - Microplate ThermoShaker for robots

Mar 182010
BioShake 3000-T elm - Microplate ThermoShaker for robots

Microplate ThermoShaker for robots Fastest and the most effective All-in-One ThermoShaker on the market Compact design with a fully integrated electronic Automatic Edge Locking Mechanism (ELM) for robotic gripping Fast and high-precision heating from ambient to 99°C Mixing up to 3,000 rpm at 2 mm orbit Homing position ± 0.1 mm RS232/USB interface Made of aluminium [...]

BioShake 3000 elm - Microplate Orbital Shaker for robots

Jan 212010
BioShake 3000 elm - Microplate Orbital Shaker for robots

Microplate Orbital Shaker for robots Fastest and the most effective All-in-One shaker on the market Compact design with a fully integrated electronic Automatic Edge Locking Mechanism (ELM) for robotic gripping 2 mm orbit for all 96, 384 and 1536 well microplates Shaking and mixing up to 3,000 rpm Homing position ± 0.1 mm RS232/USB interface Made [...]

Optimized temperature control in Eppendorf® tubes

Jan 212010

BioShake iQ – Temperature uniformity while heating-up processes

Movies - Optimized mixing in microplates

Dec 092009

We present video-clips of mixing processes in microplates.

Optimized temperature control in microplates

Dec 082009
Optimized temperature control in microplates

BioShake iQ – Temperature uniformity while heating-up processes

Optimization of mixing results in microplates

Dec 072009
Optimization of mixing results in microplates

We present video-clips of mixing processes in microplates. Fast and gentle mixing of samples in 384- and 1536-well microplates can only be succeed with orbital shaking and frequencies >2000 rpm. Watch the videoclip… The ideal mixing frequency is dependent on the well size, the filling volume and the viscosity of the solution. Watch the videoclip… [...]

Optimization of mixing results in microplates - Movie 5

Dec 072009
Optimization of mixing results in microplates - Movie 5

3D-Shake-Control prevents contamination between wells.

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